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Standard is weekly same-day dosing, but timing within that day matters significantly

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Immunoblot assays Tissue samples were crushed on dry ice to obtain homogenous aliquots and then lysed in RIPA buffer (Thermo Scientific #89900) containing Halt protease & phosphatase inhibitor (Thermo Scientific #1861280)

doi: 10.1111/ppe.12414

The latter are coated with enzyme-sensitive linkers or coatings that are responsive to MMPs and disintegrate to release the entrapped antioxidants at the tumour site